8BN5

Crystal structure of the ligand-binding domain (LBD) of human iGluR Delta-1 (GluD1) in complex with GABA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	45.838, 66.967, 175.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.350 - 1.900
R-factor	0.186
Rwork	0.184
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v3u
RMSD bond length	0.004
RMSD bond angle	0.678
Data reduction software	XDS (Feb 5, 2021 (BUILT 20210205))
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0270)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.710	2.030
High resolution limit [Å]	1.900	1.900
Rmerge	0.042	1.719
Rpim	0.600	0.600
Number of reflections	37143	1837
<I/σ(I)>	10.3	1.2
Completeness [%]	93.9	51.5
Redundancy	10.6	9.1
CC(1/2)	0.996	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	30% (w/v) PEG 4000, 100 mM HEPES pH 7.5, 200 mM CaCl2