8BN5
Crystal structure of the ligand-binding domain (LBD) of human iGluR Delta-1 (GluD1) in complex with GABA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.838, 66.967, 175.413 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.350 - 1.900 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v3u |
RMSD bond length | 0.004 |
RMSD bond angle | 0.678 |
Data reduction software | XDS (Feb 5, 2021 (BUILT 20210205)) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0270) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.710 | 2.030 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.042 | 1.719 |
Rpim | 0.600 | 0.600 |
Number of reflections | 37143 | 1837 |
<I/σ(I)> | 10.3 | 1.2 |
Completeness [%] | 93.9 | 51.5 |
Redundancy | 10.6 | 9.1 |
CC(1/2) | 0.996 | 0.525 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 30% (w/v) PEG 4000, 100 mM HEPES pH 7.5, 200 mM CaCl2 |