2V3U

Structure of the ligand-binding core of the ionotropic glutamate receptor-like GluRdelta2 in complex with D-serine

Summary for 2V3U

• Entry DOI: 10.2210/pdb2v3u/pdb
• Related: 2V3T
• Descriptor: GLUTAMATE RECEPTOR DELTA-2 SUBUNIT, D-SERINE, SODIUM ION, ... (6 entities in total)
• Functional Keywords: postsynaptic membrane, ionotropic glutamate receptors, transmembrane, membrane protein, ligand-binding core, glycoprotein, ionic channel, ion transport, membrane, receptor, d-serine, transport
• Biological source: RATTUS NORVEGICUS (RAT)
• Total number of polymer chains: 1
• Total formula weight: 30217.37

Authors

Naur, P.,Vestergaard, B.,Gajhede, M.,Kastrup, J.S. (deposition date: 2007-06-22, release date: 2007-08-07, Last modification date: 2023-12-13)

Primary citation

Naur, P.,Hansen, K.B.,Kristensen, A.S.,Dravid, S.M.,Pickering, D.S.,Olsen, L.,Vestergaard, B.,Egebjerg, J.,Gajhede, M.,Traynelis, S.F.,Kastrup, J.S.
Ionotropic Glutamate-Like Receptor {Delta}2 Binds D-Serine and Glycine.
Proc.Natl.Acad.Sci.USA, 104:14116-, 2007

PubMed Abstract: The orphan glutamate-like receptor GluRdelta2 is predominantly expressed in Purkinje cells of the central nervous system. The classification of GluRdelta2 to the ionotropic glutamate receptor family is based on sequence similarities, because GluRdelta2 does not form functional homomeric glutamate-gated ion channels in transfected cells. Studies in GluRdelta2(-/-) knockout mice as well as in mice with naturally occurring mutations in the GluRdelta2 gene have demonstrated an essential role of GluRdelta2 in cerebellar long-term depression, motor learning, motor coordination, and synaptogenesis. However, the lack of a known agonist has hampered investigations on the function of GluRdelta2. In this study, the ligand-binding core of GluRdelta2 (GluRdelta2-S1S2) was found to bind neutral amino acids such as D-serine and glycine, as demonstrated by isothermal titration calorimetry. Direct evidence for binding of D-serine and structural rearrangements in the binding cleft of GluRdelta2-S1S2 is provided by x-ray structures of GluRdelta2-S1S2 in its apo form and in complex with D-serine. Functionally, D-serine and glycine were shown to inactivate spontaneous ion-channel conductance in GluRdelta2 containing the lurcher mutation (EC(50) values, 182 and 507 microM, respectively). These data demonstrate that the GluRdelta2 ligand-binding core is capable of binding ligands and that cleft closure of the ligand-binding core can induce conformational changes that alter ion permeation.

PubMed: 17715062
DOI: 10.1073/PNAS.0703718104

Experimental method

X-RAY DIFFRACTION (1.74 Å)