8BFF
Human PPARgamma in complex with MINCH bound to the AF-2 sub-pocket
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 31 |
Unit cell lengths | 92.181, 92.181, 89.509 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.510 | 2.650 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.180 | 7.145 |
Rmeas | 0.191 | |
Rpim | 2.607 | |
Number of reflections | 26138 | 1318 |
<I/σ(I)> | 8.8 | 0.4 |
Completeness [%] | 99.7 | 99.2 |
Redundancy | 9.8 | 8.3 |
CC(1/2) | 0.998 | 0.357 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292.15 | 0.1 M Tris-HCl pH 8.5 30% PEG 2000 0.2 M Lithium sulfate 0.01 M guanidine hydrochloride |