8B7H
Crystal structure of human Gremlin-1 in complex with Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2014-09-15 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9786 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 65.740, 80.650, 252.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.960 - 1.950 |
R-factor | 0.2656 |
Rwork | 0.265 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jph |
RMSD bond length | 0.011 |
RMSD bond angle | 1.560 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.960 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.051 | 0.604 |
Number of reflections | 48941 | 2672 |
<I/σ(I)> | 18.3 | |
Completeness [%] | 99.3 | |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M citric acid pH4, 1M lithium chloride and 27% polyethylene glycol (PEG) 6000 |