8AV2
Crystal structure for the FnIII module of mouse LEP-R in complex with the anti-LEP-R nanobody VHH-4.80
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-05-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.972418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.192, 85.876, 144.689 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.420 - 1.750 |
R-factor | 0.1953 |
Rwork | 0.193 |
R-free | 0.23330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ohq AF-P48356-F1-model_v1 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.930 |
Data reduction software | XDS (VERSION Jan 10, 2022 BUILT=20220220) |
Data scaling software | Aimless (version 0.7.7) |
Phasing software | PHASER (version 2.8.3) |
Refinement software | BUSTER (2.10.4 (3-FEB-2022)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 1.860 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmeas | 0.164 | 2.066 |
Number of reflections | 67680 | 10778 |
<I/σ(I)> | 10.48 | 0.86 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 11.9 | 11.5 |
CC(1/2) | 0.998 | 0.326 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2 M ammonium sulphate 0.1 M Na cacodylate pH 5.6 30% PEG Smear Medium |