8AJ8

Structure of p110 gamma bound to the p84 regulatory subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	322.347, 166.598, 255.794
Unit cell angles	90.00, 114.02, 90.00

Refinement procedure

Resolution	90.290 - 8.500
R-factor	0.2857
Rwork	0.280
R-free	0.33990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7mez
RMSD bond length	0.004
RMSD bond angle	0.635
Data reduction software	MOSFLM (7.0.3)
Data scaling software	SCALA (6.0)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.300	8.890
High resolution limit [Å]	8.500	8.500
Rmerge	0.122	0.390
Rmeas	0.129	0.463
Rpim	0.066	0.228
Number of reflections	10835	1187
<I/σ(I)>	6.8	4.4
Completeness [%]	99.9
Redundancy	5.8
CC(1/2)	0.990	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	Optimized crystals were grown from a crystallization solution containing 16% EDO_P8K (20% w/v PEG 8000, 40% v/v ethylene glycol), 0.06 M amino acids (0.2 M sodium L-glutamate, 0.2 M DL-alanine, 0.2 M glycine, 0.2 M DL-lysine, 0.2 M DL-serine), 0.08 M buffer 2 pH7.5 (0.5 M HEPES, 0.5 M MOPS), 0.4 M Na/K phosphate pH 6.3.