8A58

X-ray structure of TRIM21 RING E3 ligase in complex with E2 enzyme Ube2W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.984004
Spacegroup name	P 1 21 1
Unit cell lengths	62.820, 75.482, 63.835
Unit cell angles	90.00, 119.31, 90.00

Refinement procedure

Resolution	29.460 - 2.250
R-factor	0.2174
Rwork	0.215
R-free	0.25880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5olm
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.460	2.330
High resolution limit [Å]	2.250	2.250
Number of reflections	23652	2357
<I/σ(I)>	16.49
Completeness [%]	95.3
Redundancy	3.3
CC(1/2)	0.999	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.1 M Bicine pH 9.0, 5% PEG 6000, 0.1M TCEP hydrochloride