8X3S

Crystal structure of human WDR5 in complex with PTEN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	46.460, 63.750, 92.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.190 - 1.870
Rwork	0.224
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.190	1.910
High resolution limit [Å]	1.870	1.870
Number of reflections	22670	1831
<I/σ(I)>	10.5
Completeness [%]	97.0
Redundancy	1.8
CC(1/2)	0.994	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M lithium sulfate monohydrate, 0.1 M HEPES at pH 7.5, 25% w/v polyethylene glycol 3350