8TRT

Structure of the EphA2 CRD bound to FabS1CE_C1, monoclinic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	57.710, 182.135, 74.380
Unit cell angles	90.00, 92.93, 90.00

Refinement procedure

Resolution	68.780 - 3.000
R-factor	0.1966
Rwork	0.194
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8trs
RMSD bond length	0.011
RMSD bond angle	1.211
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.780	3.040
High resolution limit [Å]	2.900	2.900
Rmerge	0.195	0.874
Rmeas	0.211	0.959
Rpim	0.079	0.384
Number of reflections	32852	4372
<I/σ(I)>	5.7	1.9
Completeness [%]	97.0	97
Redundancy	6.6	5.8
CC(1/2)	0.989	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	298	200 mM sodium chloride, 100 mM Bis-Tris, 20% PEG3350