8QZZ
Crystal structure of human eIF2 alpha-gamma complexed with PPP1R15A_420-452
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-29 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.6199 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 120.594, 120.594, 158.259 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.150 - 3.350 |
R-factor | 0.24368 |
Rwork | 0.241 |
R-free | 0.29915 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.911 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.170 | 3.620 |
High resolution limit [Å] | 3.350 | 3.350 |
Rmerge | 0.271 | 2.618 |
Rmeas | 0.280 | 2.714 |
Rpim | 0.113 | 1.109 |
Number of reflections | 17421 | 3497 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 99.9 | |
Redundancy | 12.3 | |
CC(1/2) | 0.996 | 0.521 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 11-12% PEG 6000, 0.1M Tris-HCl pH8.5 supplemented with amino acids |