8OW2
Crystal structure of the p110alpha catalytic subunit from homo sapiens in complex with activator 1938
Replaces: 8BFVExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.720, 134.880, 144.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.460 - 2.570 |
R-factor | 0.2158 |
Rwork | 0.213 |
R-free | 0.27140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.576 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.21rc1_4924) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 135.000 | 2.610 |
High resolution limit [Å] | 2.570 | 2.570 |
Rmeas | 0.140 | 2.600 |
Number of reflections | 37543 | 1832 |
<I/σ(I)> | 11.8 | |
Completeness [%] | 100.0 | |
Redundancy | 8.7 | |
CC(1/2) | 0.990 | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 12.5% (w/v) PEG 4K, 20% (v/v) 1,2,6-hexanetriol, 50 mM Polyamines, 0.1 M MOPSO/bis-tris pH 6.5 |