8GU7

Selective targeting of the Beclin 2-Atg14L coiled coil complex by stapled peptides promotes autophagy and endolysosomal trafficking of GPCRs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	289
Detector technology	CCD
Collection date	2017-04-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 43 21 2
Unit cell lengths	72.500, 72.500, 68.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.110 - 2.600
R-factor	0.2183
Rwork	0.217
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8gt9
Data reduction software	SCALEIT
Data scaling software	SCALA
Phasing software	Arcimboldo
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.260	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	6736	951
<I/σ(I)>	14.1
Completeness [%]	99.8	100
Redundancy	12.2
CC(1/2)	0.995	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	1.8 M Ammonium citrate tribasic, pH 7.0