8FFE
Crystal structure of LRP6 E1E2 domains bound to YW210.09 Fab and engineered XWnt8 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-14 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.212, 91.385, 104.055 |
| Unit cell angles | 90.00, 103.67, 90.00 |
Refinement procedure
| Resolution | 44.370 - 1.720 |
| R-factor | 0.1873 |
| Rwork | 0.185 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.974 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.370 | 1.781 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmeas | 0.104 | 2.243 |
| Rpim | 0.039 | 0.856 |
| Number of reflections | 109415 | 6719 |
| <I/σ(I)> | 10.49 | 0.64 |
| Completeness [%] | 90.6 | 55.47 |
| Redundancy | 7.1 | 6.7 |
| CC(1/2) | 0.997 | 0.426 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100 mM SPG (succinate/phosphate/glycine buffer), 25% PEG 1500 |
