8D5B

Crystal structure of human METTL1 in complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	P 61
Unit cell lengths	86.034, 86.034, 65.483
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.250 - 1.930
R-factor	0.1823
Rwork	0.181
R-free	0.21220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ckk
RMSD bond length	0.006
RMSD bond angle	0.951
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.250	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.242	3.220
Rpim	0.057	1.030
Number of reflections	20856	1034
<I/σ(I)>	16.24	1.3
Completeness [%]	100.0	99.9
Redundancy	18.1	10.5
CC(1/2)	0.996	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	50 mM Sodium cacodylate pH 5.7, 1.5 M Lithium sulfate, 10 mM Magnesium sulfate