8B7H

Crystal structure of human Gremlin-1 in complex with Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	80
Detector technology	PIXEL
Collection date	2014-09-15
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	65.740, 80.650, 252.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.960 - 1.950
R-factor	0.2656
Rwork	0.265
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jph
RMSD bond length	0.011
RMSD bond angle	1.560
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.960	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.051	0.604
Number of reflections	48941	2672
<I/σ(I)>	18.3
Completeness [%]	99.3
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M citric acid pH4, 1M lithium chloride and 27% polyethylene glycol (PEG) 6000