8AV2

Crystal structure for the FnIII module of mouse LEP-R in complex with the anti-LEP-R nanobody VHH-4.80

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.972418
Spacegroup name	P 21 21 21
Unit cell lengths	53.192, 85.876, 144.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.420 - 1.750
R-factor	0.1953
Rwork	0.193
R-free	0.23330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ohq AF-P48356-F1-model_v1
RMSD bond length	0.008
RMSD bond angle	0.930
Data reduction software	XDS (VERSION Jan 10, 2022 BUILT=20220220)
Data scaling software	Aimless (version 0.7.7)
Phasing software	PHASER (version 2.8.3)
Refinement software	BUSTER (2.10.4 (3-FEB-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	1.860
High resolution limit [Å]	1.750	1.750
Rmeas	0.164	2.066
Number of reflections	67680	10778
<I/σ(I)>	10.48	0.86
Completeness [%]	100.0	99.9
Redundancy	11.9	11.5
CC(1/2)	0.998	0.326

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.2 M ammonium sulphate 0.1 M Na cacodylate pH 5.6 30% PEG Smear Medium