8AJ8
Structure of p110 gamma bound to the p84 regulatory subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 322.347, 166.598, 255.794 |
Unit cell angles | 90.00, 114.02, 90.00 |
Refinement procedure
Resolution | 90.290 - 8.500 |
R-factor | 0.2857 |
Rwork | 0.280 |
R-free | 0.33990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7mez |
RMSD bond length | 0.004 |
RMSD bond angle | 0.635 |
Data reduction software | MOSFLM (7.0.3) |
Data scaling software | SCALA (6.0) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.300 | 8.890 |
High resolution limit [Å] | 8.500 | 8.500 |
Rmerge | 0.122 | 0.390 |
Rmeas | 0.129 | 0.463 |
Rpim | 0.066 | 0.228 |
Number of reflections | 10835 | 1187 |
<I/σ(I)> | 6.8 | 4.4 |
Completeness [%] | 99.9 | |
Redundancy | 5.8 | |
CC(1/2) | 0.990 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | Optimized crystals were grown from a crystallization solution containing 16% EDO_P8K (20% w/v PEG 8000, 40% v/v ethylene glycol), 0.06 M amino acids (0.2 M sodium L-glutamate, 0.2 M DL-alanine, 0.2 M glycine, 0.2 M DL-lysine, 0.2 M DL-serine), 0.08 M buffer 2 pH7.5 (0.5 M HEPES, 0.5 M MOPS), 0.4 M Na/K phosphate pH 6.3. |