7ZMZ

Engineered Interleukin 2 bound to CD25 receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 32 2 1
Unit cell lengths	89.046, 89.046, 116.683
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.300 - 3.200
R-factor	0.238
Rwork	0.237
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b5i
RMSD bond length	0.006
RMSD bond angle	1.165
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.330	3.420
High resolution limit [Å]	3.200	3.200
Rmerge	0.233	5.973
Rmeas	0.246	6.293
Rpim	0.078	1.974
Number of reflections	9238	1650
<I/σ(I)>	8.3	0.5
Completeness [%]	100.0	100
Redundancy	18.4	19.1
CC(1/2)	0.999	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	19% PEG 3350, 0.2 M Sodium tartrate dibasic dihydrate, 10% glycerol