7ZMZ

Engineered Interleukin 2 bound to CD25 receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Interleukin-2	polymer	146	16821.4	1	UniProt (P60568) Pfam (PF00715)	Homo sapiens (human)	IL-2,T-cell growth factor,TCGF
2	B (D)	Interleukin-2 receptor subunit alpha	polymer	258	29016.3	1	UniProt (P01589) Pfam (PF00084)	Homo sapiens (human)	IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55

Sequence modifications

A: 0 - 133 (UniProt: P60568)

PDB	External Database	Details
Gly -1	-	expression tag
His 37	Thr 57	engineered mutation
Leu 38	Arg 58	engineered mutation
Ser 41	Thr 61	engineered mutation
Tyr 42	Phe 62	engineered mutation
Gly 43	Lys 63	engineered mutation
Phe 77	Asn 97	conflict
Asn 78	Phe 98	conflict
Ala 134	-	expression tag
Ala 135	-	expression tag
Ala 136	-	expression tag
His 137	-	expression tag
His 138	-	expression tag
His 139	-	expression tag
His 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag

D: 0 - 217 (UniProt: P01589)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 218	-	expression tag
Ala 219	-	expression tag
Ala 220	-	expression tag
Leu 221	-	expression tag
Glu 222	-	expression tag
Val 223	-	expression tag
Leu 224	-	expression tag
Phe 225	-	expression tag
Gln 226	-	expression tag
Gly 227	-	expression tag
Pro 228	-	expression tag
Gly 229	-	expression tag
Ala 230	-	expression tag
Ala 231	-	expression tag
Gly 232	-	expression tag
Gly 233	-	expression tag
Gly 234	-	expression tag
Leu 235	-	expression tag
Asn 236	-	expression tag
Asp 237	-	expression tag
Ile 238	-	expression tag
Phe 239	-	expression tag
Glu 240	-	expression tag
Ala 241	-	expression tag
Gln 242	-	expression tag
Lys 243	-	expression tag
Ile 244	-	expression tag
Glu 245	-	expression tag
Trp 246	-	expression tag
His 247	-	expression tag
Glu 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45837.7
All*	Total formula weight	45837.7

*Water molecules are not included.