7ZJ3
Structure of TRIM2 RING domain in complex with UBE2D1~Ub conjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-04 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 1.2821 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.319, 123.662, 112.471 |
| Unit cell angles | 90.00, 102.80, 90.00 |
Refinement procedure
| Resolution | 54.970 - 2.530 |
| R-factor | 0.2129 |
| Rwork | 0.210 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fer |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.833 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.675 | 2.620 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Number of reflections | 51031 | 5126 |
| <I/σ(I)> | 4.11 | 0.38 |
| Completeness [%] | 99.8 | 99.71 |
| Redundancy | 6.5 | 6.2 |
| CC(1/2) | 0.990 | 0.537 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5 10% (w/v) PEG 8K |
