7ZJ3

Structure of TRIM2 RING domain in complex with UBE2D1~Ub conjugate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-04
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.2821
Spacegroup name	P 1 21 1
Unit cell lengths	57.319, 123.662, 112.471
Unit cell angles	90.00, 102.80, 90.00

Refinement procedure

Resolution	54.970 - 2.530
R-factor	0.2129
Rwork	0.210
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fer
RMSD bond length	0.005
RMSD bond angle	0.833
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.675	2.620
High resolution limit [Å]	2.530	2.530
Number of reflections	51031	5126
<I/σ(I)>	4.11	0.38
Completeness [%]	99.8	99.71
Redundancy	6.5	6.2
CC(1/2)	0.990	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES pH 7.5 10% (w/v) PEG 8K