7Z3J
Structure of crystallisable rat Phospholipase C gamma 1 in complex with inositol 1,4,5-trisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.88560 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.760, 82.440, 230.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.290 - 2.000 |
R-factor | 0.2254 |
Rwork | 0.224 |
R-free | 0.25640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bpc |
RMSD bond length | 0.009 |
RMSD bond angle | 1.006 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP (11.7.03) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 115.100 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.076 | |
Number of reflections | 93254 | 28825 |
<I/σ(I)> | 10.83 | |
Completeness [%] | 98.8 | |
Redundancy | 13.5 | |
CC(1/2) | 0.999 | 0.234 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 18.7 % PEG 3350, 0.1 M CBTP |