7Z2P

Tubulin-nocodazole complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97976
Spacegroup name	P 21 21 21
Unit cell lengths	104.732, 158.090, 179.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.370 - 2.000
R-factor	0.1799
Rwork	0.178
R-free	0.20880
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.370	2.072
High resolution limit [Å]	2.000	2.000
Rmerge	0.123
Rmeas	0.128
Rpim	0.035
Number of reflections	200305	19421
<I/σ(I)>	14.63
Completeness [%]	99.8
Redundancy	13.5
CC(1/2)	0.999	0.393

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine