7Z2P

Tubulin-nocodazole complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Tubulin alpha-1B chain	polymer	451	50204.4	2	UniProt (P81947) Pfam (PF00091) Pfam (PF03953)	Bos taurus (cattle)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B, D (B, D)	Tubulin beta-2B chain	polymer	445	49999.9	2	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953)	Bos taurus (cattle)
3	E (E)	Stathmin-4	polymer	143	16844.2	1	UniProt (P63043) Pfam (PF00836)	Rattus norvegicus (Norway rat)	Stathmin-like protein B3,RB3
4	F (F)	Tubulin beta-2B chain	polymer	384	44378.5	1	UniProt (E1BQ43)	Gallus gallus (chicken)
5	G, M (A, C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
6	H, J, N, P, T (A, B, C, D, F)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
7	I, O (B, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
8	K, Q (B, D)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
9	L, R (B, D)	nocodazole	non-polymer		301.3	2	Chemie (NW6)
10	S (F)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
11	U, V, W, X, Y... (A, B, C, D, E...)	water	water		18.0	777	Chemie (HOH)

Sequence modifications

E: 5 - 145 (UniProt: P63043)

PDB	External Database	Details
Met 3	-	initiating methionine
Ala 4	-	expression tag

F: 1 - 378 (UniProt: E1BQ43)

PDB	External Database	Details
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	261631.3
Non-Polymers*	Number of molecules	14
	Total formula weight	3552.6
All*	Total formula weight	265183.9

*Water molecules are not included.