7YXR
Crystal structure of mutant AncGR2-LBD (Y545A) bound to dexamethasone and SHP coregulator fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 |
Unit cell lengths | 107.899, 107.899, 134.012 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.510 - 2.500 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ufs |
RMSD bond length | 0.007 |
RMSD bond angle | 1.564 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.400 | 9.010 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmeas | 0.122 | 0.636 |
Rpim | 0.081 | 0.360 |
Number of reflections | 30280 | 3425 |
<I/σ(I)> | 6.5 | 1.9 |
Completeness [%] | 99.0 | 99.5 |
Redundancy | 2.9 | 2.9 |
CC(1/2) | 0.993 | 0.621 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.1 M PIPES, pH 7.0, 0.1 M ammonium acetate, 2.5 M sodium formate |