7YXB
MHC-II dynamics are maintained in HLA-DR allotypes to ensure catalyzed peptide exchange
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 96.891, 111.485, 212.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.081 - 2.095 |
R-factor | 0.1881 |
Rwork | 0.187 |
R-free | 0.22210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x5x |
RMSD bond length | 0.005 |
RMSD bond angle | 0.741 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.081 | 2.220 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmeas | 0.016 | 0.151 |
Number of reflections | 67120 | 10573 |
<I/σ(I)> | 9.69 | 1.28 |
Completeness [%] | 99.0 | |
Redundancy | 4.56 | |
CC(1/2) | 0.996 | 0.491 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.2M Ammoniumcitrate |