7YX9

MHC-II dynamics are maintained in HLA-DR allotypes to ensure catalyzed peptide exchange

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91841
Spacegroup name	P 1 21 1
Unit cell lengths	57.712, 120.803, 68.236
Unit cell angles	90.00, 108.84, 90.00

Refinement procedure

Resolution	40.510 - 1.760
R-factor	0.1681
Rwork	0.167
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x5x
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.510	1.860
High resolution limit [Å]	1.760	1.760
Rmeas	0.099	0.761
Number of reflections	328677	22740
<I/σ(I)>	11.39
Completeness [%]	98.5	98
Redundancy	3.8
CC(1/2)	0.998	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% PEG3350, 0.2M MgCl2, 0.1M Hepes pH 7.5