7YV4
Crystal structure of human UCHL3 in complex with Farrerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-16 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.054, 63.873, 77.161 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.600 - 1.580 |
R-factor | 0.1806 |
Rwork | 0.179 |
R-free | 0.20630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6isu |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.600 | 1.630 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.158 | 0.158 |
Number of reflections | 32507 | 32507 |
<I/σ(I)> | 20.67 | |
Completeness [%] | 96.8 | |
Redundancy | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 1.4M Sodium Citrate tribasic dihydrate, 0.1M HEPES Sodium pH 7.0 |