7YV4

Crystal structure of human UCHL3 in complex with Farrerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-16
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	47.054, 63.873, 77.161
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.600 - 1.580
R-factor	0.1806
Rwork	0.179
R-free	0.20630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6isu
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.600	1.630
High resolution limit [Å]	1.580	1.580
Rmerge	0.158	0.158
Number of reflections	32507	32507
<I/σ(I)>	20.67
Completeness [%]	96.8
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	1.4M Sodium Citrate tribasic dihydrate, 0.1M HEPES Sodium pH 7.0