7YV4

Crystal structure of human UCHL3 in complex with Farrerol

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004843	molecular_function	cysteine-type deubiquitinase activity
A	0005515	molecular_function	protein binding
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006508	biological_process	proteolysis
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0008233	molecular_function	peptidase activity
A	0008234	molecular_function	cysteine-type peptidase activity
A	0016567	biological_process	protein ubiquitination
A	0016579	biological_process	protein deubiquitination
A	0019784	molecular_function	deNEDDylase activity
A	0030163	biological_process	protein catabolic process
A	0043130	molecular_function	ubiquitin binding
A	0043687	biological_process	post-translational protein modification

Functional Information from PROSITE/UniProt

site_id	PS00140
Number of Residues	17
Details	UCH_1 Ubiquitin carboxyl-terminal hydrolase family 1 cysteine active-site. QtisNACGtigLIHAIA

Chain	Residue	Details
A	GLN89-ALA105

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:19154770, ECO:0000269|PubMed:20380862, ECO:0000269|PubMed:9790970

Chain	Residue	Details
A	CYS95

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000250|UniProtKB:P09936

Chain	Residue	Details
A	HIS169

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site_id	SWS_FT_FI3
Number of Residues	1
Details	SITE: Important for enzyme activity => ECO:0000250|UniProtKB:P09936

Chain	Residue	Details
A	ASP184

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	SER130

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 597

Chain	Residue	Details
A	CYS95	covalent catalysis, proton shuttle (general acid/base)
A	GLN89	electrostatic stabiliser
A	HIS169	proton shuttle (general acid/base)
A	ASP184	electrostatic stabiliser

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