7YQE
Structure of human SRC regulatory domains in complex with the C-terminal PRRP motifs of GPR54.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 83 |
| Detector technology | PIXEL |
| Collection date | 2020-11-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978530 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 95.245, 95.245, 122.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.970 - 3.500 |
| R-factor | 0.2703 |
| Rwork | 0.268 |
| R-free | 0.29460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2src |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.900 | 3.599 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.160 | 1.536 |
| Rmeas | 0.167 | 1.579 |
| Rpim | 0.039 | 0.365 |
| Number of reflections | 8144 | 395 |
| <I/σ(I)> | 12.7 | 2.4 |
| Completeness [%] | 97.4 | 100 |
| Redundancy | 19.1 | 18.4 |
| CC(1/2) | 0.992 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1% v/v 1,4 Dioxane, 0.1 M Tris pH 7.5, 12.5% w/v Polyethylene glycol 3,350 |
