7X1O
Homo sapiens Prolyl-tRNA Synthetase (HsPRS) in Complex with double inhibitors HF and L95
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-30 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.030, 93.730, 87.090 |
| Unit cell angles | 90.00, 109.62, 90.00 |
Refinement procedure
| Resolution | 33.519 - 2.040 |
| R-factor | 0.1964 |
| Rwork | 0.194 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7x09 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15rc1_3423) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.870 | 2.080 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmeas | 0.077 | 2.377 |
| Number of reflections | 69421 | 3469 |
| <I/σ(I)> | 13.3 | 0.8 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 7 | 7.2 |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES PH 7.5, 1.5 M CACL2, 20% PEG 3350 |
