7VI0

Crystal structure of EP300 HAT domain in complex with compound 11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-10-02
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.541800
Spacegroup name	P 1
Unit cell lengths	43.555, 88.361, 90.655
Unit cell angles	116.52, 94.38, 91.36

Refinement procedure

Resolution	19.720 - 2.100
R-factor	0.2011
Rwork	0.199
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bhw
Data reduction software	CrystalClear
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.720	19.720	2.230
High resolution limit [Å]	2.100	6.030	2.100
Rmerge	0.128	0.036	1.312
Rmeas	0.148	0.043	1.521
Total number of observations	262252
Number of reflections	66772	2853	10166
<I/σ(I)>	8.75	30.28	1.01
Completeness [%]	94.8	95.7	90.3
Redundancy	3.928	3.767	3.834
CC(1/2)	0.996	0.998	0.501

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	12% PEG3350, 0.1 M HEPES (pH 7.0)