7V4G

Crystal structure of human ALKBH5 in complex with m6A-containing ssRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-15
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 32
Unit cell lengths	76.474, 76.474, 106.347
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.460 - 2.100
R-factor	0.1905
Rwork	0.185
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nj4
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.460	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.211	1.121
Number of reflections	40605	4132
<I/σ(I)>	8.61	1.4
Completeness [%]	100.0
Redundancy	5
CC(1/2)	0.987	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M sodium sulfate decahydrate, 20% (w/v) PEG 3350