7V0E
Crystal structure of the macro-oligomeric form of DNMT3B methyltransferase domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9195 |
| Spacegroup name | P 32 |
| Unit cell lengths | 164.393, 164.393, 209.175 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.288 - 3.270 |
| R-factor | 0.206233223941 |
| Rwork | 0.206 |
| R-free | 0.23458 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6u8p |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.868 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.370 | 3.330 |
| High resolution limit [Å] | 3.270 | 3.270 |
| Rmerge | 0.983 | |
| Number of reflections | 93113 | 4439 |
| <I/σ(I)> | 8.2 | 1.3 |
| Completeness [%] | 95.3 | 91.5 |
| Redundancy | 3 | 2.9 |
| CC(1/2) | 0.980 | 0.360 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.1 M MES pH6.5, 1.4 M Ammonium sulfate, 0.2 mM AdoHcy and 8% v/v 1,4-Dioxane |
