7UMB

NanoBRET tracer Tram-bo bound to a KSR2-MEK1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-12-06
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1
Spacegroup name	P 61 2 2
Unit cell lengths	139.500, 139.500, 220.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.710 - 3.231
R-factor	0.2306
Rwork	0.229
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y4i
RMSD bond length	0.012
RMSD bond angle	1.401
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.710	3.310
High resolution limit [Å]	3.231	3.231
Rmeas	0.166
Number of reflections	20907	1508
<I/σ(I)>	13.86
Completeness [%]	99.0	100
Redundancy	20.6
CC(1/2)	0.492	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 2000, MES, pH 6.5, Magnesium acetate