7U0L
Crystal structure of the CCoV-HuPn-2018 RBD (domain B) in complex with canine APN
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-12-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.988 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 101.431, 107.329, 239.229 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.960 - 3.300 |
R-factor | 0.2167 |
Rwork | 0.215 |
R-free | 0.24010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f5c |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.610 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.065 | |
Number of reflections | 40080 | 4470 |
<I/σ(I)> | 6.5 | |
Completeness [%] | 100.0 | |
Redundancy | 13.4 | |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M Na2HPO4-citric acid pH 4.2, 1,6-,0.4 M Na2HPO4/K2HPO4 |