7U0L

Crystal structure of the CCoV-HuPn-2018 RBD (domain B) in complex with canine APN

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Aminopeptidase N	polymer	908	103563.3	1	UniProt (P79143) Pfam (PF17900) Pfam (PF01433) Pfam (PF11838)	Canis lupus familiaris (dog)	AP-N,cAPN,Alanyl aminopeptidase,Aminopeptidase M,AP-M,Microsomal aminopeptidase
2	B (B)	Spike glycoprotein	polymer	182	20211.4	1	UniProt (A0A8E6CMP0)	Coronaviridae sp.	S glycoprotein,E2,Peplomer protein
3	C (C)	alpha-D-mannopyranose-(1-3)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB
4	D, L (D, L)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	2	In PDB GlyTouCan (G22768VO)
5	E (E)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1057.0	1	In PDB GlyTouCan (G82348BZ)
6	F, I (F, I)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
7	G, K (G, K)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
8	H (H)	alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	1	In PDB GlyTouCan (G00850LT)
9	J, M (J, M)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		894.8	2	In PDB GlyTouCan (G00395TQ)
10	N (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
11	O, P (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
12	Q (A)	water	water		18.0	65	Chemie (HOH)

Sequence modifications

A: 72 - 973 (UniProt: P79143)

PDB	External Database	Details
Thr 71	-	expression tag
Gly 974	-	expression tag
Gly 975	-	expression tag
Ser 976	-	expression tag

B: 523 - 682 (UniProt: A0A8E6CMP0)

PDB	External Database	Details
Thr 522	-	expression tag
Gly 672	-	insertion
Ser 673	-	insertion
Ser 674	-	insertion
Gly 675	-	insertion
Gly 676	-	insertion
Asn 680	His 675	conflict
Ile 682	Leu 677	conflict
Phe 683	-	expression tag
Glu 684	-	expression tag
Ala 685	-	expression tag
Gln 686	-	expression tag
Lys 687	-	expression tag
Ile 688	-	expression tag
Glu 689	-	expression tag
Trp 690	-	expression tag
His 691	-	expression tag
Glu 692	-	expression tag
Gly 693	-	expression tag
Gly 694	-	expression tag
Ser 695	-	expression tag
His 696	-	expression tag
His 697	-	expression tag
His 698	-	expression tag
His 699	-	expression tag
His 700	-	expression tag
His 701	-	expression tag
His 702	-	expression tag
His 703	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	123774.6
Branched	Number of molecules	11
	Total formula weight	8511.8
Non-Polymers*	Number of molecules	3
	Total formula weight	507.8
All*	Total formula weight	132794.2

*Water molecules are not included.