7SZD

Crystal Structure Analysis of human PRPK complex with a compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	65.578, 79.038, 100.889
Unit cell angles	90.00, 105.61, 90.00

Refinement procedure

Resolution	97.170 - 2.050
R-factor	0.2233
Rwork	0.221
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3enp 4ww5
RMSD bond length	0.010
RMSD bond angle	1.476
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.170	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.062
Rpim	0.040
Number of reflections	28212	80
<I/σ(I)>	10.3
Completeness [%]	89.9
Redundancy	3.4
CC(1/2)	0.998	0.226

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100mM HEPES pH 7.5, 12% PEG 5000 MME, 10mM TCEP