7S7J
Structure of Human SPASTIN-IST1 complex.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-13 |
Detector | CS-PAD CXI-1 |
Wavelength(s) | 0.97946 |
Spacegroup name | I 41 |
Unit cell lengths | 79.614, 79.614, 36.331 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.050 - 1.150 |
R-factor | 0.1491 |
Rwork | 0.149 |
R-free | 0.15720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eab |
RMSD bond length | 0.007 |
RMSD bond angle | 0.907 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.050 | 1.170 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.103 | 1.647 |
Rmeas | 0.107 | |
Rpim | 0.029 | |
Number of reflections | 74704 | 1425 |
<I/σ(I)> | 16.4 | 4 |
Completeness [%] | 94.2 | 72.2 |
Redundancy | 13.6 | 12.3 |
CC(1/2) | 0.998 | 0.865 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 278 | 40% v/v PEG 300, 100 mM Sodium cacodylate / Hydrochloric acid pH=6.5, 200 mM Calcium Acetate |