7S7J

Structure of Human SPASTIN-IST1 complex.

Summary for 7S7J

• Entry DOI: 10.2210/pdb7s7j/pdb
• Descriptor: Spastin, IST1 homolog, TETRAETHYLENE GLYCOL, ... (7 entities in total)
• Functional Keywords: cytokinesis, mit, aaa-atpase, protein transport
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 12935.21

Authors

Skalicky, J.J.,Sundquist, W.I. (deposition date: 2021-09-16, release date: 2022-09-28, Last modification date: 2023-10-18)

Primary citation

Wenzel, D.M.,Mackay, D.R.,Skalicky, J.J.,Paine, E.L.,Miller, M.S.,Ullman, K.S.,Sundquist, W.I.
Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission.
Elife, 11:-, 2022

PubMed Abstract: The 12 related human ESCRT-III proteins form filaments that constrict membranes and mediate fission, including during cytokinetic abscission. The C-terminal tails of polymerized ESCRT-III subunits also bind proteins that contain Microtubule-Interacting and Trafficking (MIT) domains. MIT domains can interact with ESCRT-III tails in many different ways to create a complex binding code that is used to recruit essential cofactors to sites of ESCRT activity. Here, we have comprehensively and quantitatively mapped the interactions between all known ESCRT-III tails and 19 recombinant human MIT domains. We measured 228 pairwise interactions, quantified 60 positive interactions, and discovered 18 previously unreported interactions. We also report the crystal structure of the SPASTIN MIT domain in complex with the IST1 C-terminal tail. Three MIT enzymes were studied in detail and shown to: (1) localize to cytokinetic midbody membrane bridges through interactions with their specific ESCRT-III binding partners (SPASTIN-IST1, KATNA1-CHMP3, and CAPN7-IST1), (2) function in abscission (SPASTIN, KATNA1, and CAPN7), and (3) function in the 'NoCut' abscission checkpoint (SPASTIN and CAPN7). Our studies define the human MIT-ESCRT-III interactome, identify new factors and activities required for cytokinetic abscission and its regulation, and provide a platform for analyzing ESCRT-III and MIT cofactor interactions in all ESCRT-mediated processes.

PubMed: 36107470
DOI: 10.7554/eLife.77779

Experimental method

X-RAY DIFFRACTION (1.15 Å)