7RM4
Neoantigen p53R175H-specific TCR 6-11 binds to p53R175H-HLA-A2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 127.226, 55.144, 304.877 |
Unit cell angles | 90.00, 98.74, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.319 | 3.450 |
High resolution limit [Å] | 3.330 | 3.330 |
Number of reflections | 60837 | 4526 |
<I/σ(I)> | 10.3 | |
Completeness [%] | 92.6 | |
Redundancy | 4.8 | |
CC(1/2) | 0.992 | 0.737 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% (w/v) PEG 3350, 0.1 M Bis-Tris propane (pH 8.5), and 0.2 M Potassium thiocyanate |