7RCH
Crystal structure of NS1-ED of Vietnam influenza A virus in complex with the p85-beta-iSH2 domain of human PI3K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-01-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.440, 92.840, 67.120 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.2444 |
| Rwork | 0.243 |
| R-free | 0.27620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ox7 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.447 |
| Data reduction software | MOSFLM |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.820 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 13030 | 2318 |
| <I/σ(I)> | 4 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.52 | |
| CC(1/2) | 0.975 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 278 | 20 mM sodium phosphate, pH 7.0, 80 mM sodium chloride |
