7R5L
Crystal structure of YTHDF2 with compound YLI_DC1_015
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-28 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 76.240, 76.240, 115.840 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.120 - 1.700 |
R-factor | 0.2045 |
Rwork | 0.204 |
R-free | 0.22360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rdn |
RMSD bond length | 0.006 |
RMSD bond angle | 0.829 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.120 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 22678 | 3523 |
<I/σ(I)> | 26.77 | |
Completeness [%] | 99.8 | |
Redundancy | 19.68 | |
CC(1/2) | 1.000 | 0.901 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 1.95 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.0 |