7Q15
Crystal structure of FcRn and beta-2-microglobulin in complex with IgG1-Fc-MST-HN (efgartigimod)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-22 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.89 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.429, 53.107, 195.745 |
Unit cell angles | 90.00, 92.18, 90.00 |
Refinement procedure
Resolution | 48.900 - 3.301 |
R-factor | 0.2077 |
Rwork | 0.206 |
R-free | 0.23130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n0u |
RMSD bond length | 0.005 |
RMSD bond angle | 0.810 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.901 | 3.449 |
High resolution limit [Å] | 3.146 | 3.146 |
Rmeas | 0.380 | 0.016 |
Number of reflections | 21510 | 1073 |
<I/σ(I)> | 4.2 | 1.1 |
Completeness [%] | 90.4 | |
Redundancy | 3.1 | |
CC(1/2) | 0.942 | 0.265 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 0.1 M Sodium Cacodylate pH 7 |