7PRW
The glucocorticoid receptor in complex with velsecorat, a PGC1a coactivator fragment and sgk 23bp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-07 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.96550 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.210, 122.724, 130.449 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.390 - 2.502 |
R-factor | 0.2048 |
Rwork | 0.202 |
R-free | 0.26320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g9o 4p6w |
RMSD bond length | 0.008 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | autoPROC (1.1.7) |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.385 | 2.872 |
High resolution limit [Å] | 2.502 | 2.502 |
Rmerge | 0.082 | 0.792 |
Rmeas | 0.090 | 0.878 |
Rpim | 0.036 | 0.373 |
Number of reflections | 23070 | 1154 |
<I/σ(I)> | 12.6 | 1.9 |
Completeness [%] | 93.4 | |
Redundancy | 6.3 | |
CC(1/2) | 0.999 | 0.744 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 8.6 % PEG3350, 0.3 M 1,6-hexanediol, 0.1 M guanidine hydrochloride, 2 % 2,2,2-trifluoroethanol, 0.1 M BIS-TRIS propane pH 6.5 |