7OZD

FGFR1 kinase domain (residues 458-765) with mutations C488A, C584S in complex with 34.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9159
Spacegroup name	C 1 2 1
Unit cell lengths	207.300, 57.510, 65.970
Unit cell angles	90.00, 107.44, 90.00

Refinement procedure

Resolution	22.010 - 1.820
Rwork	0.200
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a4c
RMSD bond length	0.006
RMSD bond angle	1.439
Data reduction software	xia2
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.010	1.870
High resolution limit [Å]	1.820	1.820
Rmerge	0.030	0.836
Rmeas	0.035	1.033
Rpim	0.024	0.598
Number of reflections	66115	4910
<I/σ(I)>	16.7
Completeness [%]	99.2
Redundancy	3.4
CC(1/2)	0.999	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	291	0.185M ammonium sulfate, 20% v/v ethylene glycol, 17% w/v PEG 8000, 0.1M PCPT