7OZD
FGFR1 kinase domain (residues 458-765) with mutations C488A, C584S in complex with 34.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 207.300, 57.510, 65.970 |
| Unit cell angles | 90.00, 107.44, 90.00 |
Refinement procedure
| Resolution | 22.010 - 1.820 |
| Rwork | 0.200 |
| R-free | 0.24120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a4c |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.439 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.010 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.030 | 0.836 |
| Rmeas | 0.035 | 1.033 |
| Rpim | 0.024 | 0.598 |
| Number of reflections | 66115 | 4910 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.999 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 291 | 0.185M ammonium sulfate, 20% v/v ethylene glycol, 17% w/v PEG 8000, 0.1M PCPT |
