7OS4

Crystal structure of mouse CARM1 in complex with histone H3_13-31 K18

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-24
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	74.374, 98.606, 206.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.760 - 2.540
R-factor	0.2103
Rwork	0.208
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.760	2.620
High resolution limit [Å]	2.540	2.540
Rmerge	0.129	2.284
Rmeas	0.140	2.501
Rpim	0.055	0.996
Number of reflections	49843	4042
<I/σ(I)>	8.6
Completeness [%]	97.9	87.4
Redundancy	6.3	5.5
CC(1/2)	0.999	0.475

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	1.2M Sodium malonate 0.1M MES pH 5.5 0.2M NaCl