7OJX
E2 UBE2K covalently linked to donor Ub, acceptor di-Ub, and RING E3 primed for K48-linked Ub chain synthesis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9119 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 85.835, 85.835, 167.354 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 74.340 | 74.370 | 2.440 |
High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
Rmeas | 0.060 | 0.034 | 1.102 |
Rpim | 0.020 | 0.012 | 0.370 |
Total number of observations | 246998 | ||
Number of reflections | 28670 | 1587 | 1419 |
<I/σ(I)> | 19.1 | 74.4 | 0.7 |
Completeness [%] | 100.0 | 100 | 99.9 |
Redundancy | 8.6 | 7.9 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | Crystals were grown in: 0.2 M sodium citrate, 0.1 M Bis Tris propane 7.5 and 20 % (w/v) PEG 3350. The crystallization drops were set as a 1:1 mixture of the protein complex solution and the precipitant solution. |