7OJX

E2 UBE2K covalently linked to donor Ub, acceptor di-Ub, and RING E3 primed for K48-linked Ub chain synthesis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	E3 ubiquitin-protein ligase RNF38	polymer	79	9135.3	1	UniProt (Q9H0F5) Pfam (PF13639) In PDB	Homo sapiens (Human)	RING finger protein 38,RING-type E3 ubiquitin transferase RNF38
2	B	Ubiquitin-conjugating enzyme E2 K	polymer	202	22600.8	1	UniProt (P61086) Pfam (PF00179) Pfam (PF00627) In PDB	Homo sapiens (Human)	E2 ubiquitin-conjugating enzyme K,Huntingtin-interacting protein 2,HIP-2,Ubiquitin carrier protein,Ubiquitin-conjugating enzyme E2-25 kDa,Ubiquitin-conjugating enzyme E2(25K),Ubiquitin-conjugating enzyme E2-25K,Ubiquitin-protein ligase
3	C	Polyubiquitin-B	polymer	79	8778.0	1	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (Human)
4	D	Polyubiquitin-B	polymer	76	8550.8	1	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (Human)
5	E	Polyubiquitin-B	polymer	76	8576.8	1	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (Human)
6	A	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	B	1,1'-ethane-1,2-diylbis(1H-pyrrole-2,5-dione)	non-polymer		220.2	1	Chemie (ME7)
8		water	water		18.0	50	Chemie (HOH)

Sequence modifications

A: 389 - 465 (UniProt: Q9H0F5)

PDB	External Database	Details
Gly 387	-	expression tag
Ser 388	-	expression tag

B: 1 - 200 (UniProt: P61086)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Lys 92	Cys 92	engineered mutation
Arg 97	Lys 97	engineered mutation
Cys 124	Asp 124	engineered mutation
Ser 170	Cys 170	engineered mutation

C: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

D: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Cys 48	Lys 48	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	57641.7
Non-Polymers*	Number of molecules	3
	Total formula weight	351.0
All*	Total formula weight	57992.7

*Water molecules are not included.