7OJX
E2 UBE2K covalently linked to donor Ub, acceptor di-Ub, and RING E3 primed for K48-linked Ub chain synthesis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | E3 ubiquitin-protein ligase RNF38 | polymer | 79 | 9135.3 | 1 | UniProt (Q9H0F5) Pfam (PF13639) | Homo sapiens (Human) | RING finger protein 38,RING-type E3 ubiquitin transferase RNF38 |
| 2 | B (B) | Ubiquitin-conjugating enzyme E2 K | polymer | 202 | 22600.8 | 1 | UniProt (P61086) Pfam (PF00179) Pfam (PF00627) | Homo sapiens (Human) | E2 ubiquitin-conjugating enzyme K,Huntingtin-interacting protein 2,HIP-2,Ubiquitin carrier protein,Ubiquitin-conjugating enzyme E2-25 kDa,Ubiquitin-conjugating enzyme E2(25K),Ubiquitin-conjugating enzyme E2-25K,Ubiquitin-protein ligase |
| 3 | C (C) | Polyubiquitin-B | polymer | 79 | 8778.0 | 1 | UniProt (P0CG47) Pfam (PF00240) | Homo sapiens (Human) | |
| 4 | D (D) | Polyubiquitin-B | polymer | 76 | 8550.8 | 1 | UniProt (P0CG47) Pfam (PF00240) | Homo sapiens (Human) | |
| 5 | E (E) | Polyubiquitin-B | polymer | 76 | 8576.8 | 1 | UniProt (P0CG47) Pfam (PF00240) | Homo sapiens (Human) | |
| 6 | F, G (A) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 7 | H (B) | 1,1'-ethane-1,2-diylbis(1H-pyrrole-2,5-dione) | non-polymer | 220.2 | 1 | Chemie (ME7) | |||
| 8 | I, J, K, L, M (A, B, C, D, E) | water | water | 18.0 | 50 | Chemie (HOH) |
Sequence modifications
A: 389 - 465 (UniProt: Q9H0F5)
B: 1 - 200 (UniProt: P61086)
C: 1 - 76 (UniProt: P0CG47)
D: 1 - 76 (UniProt: P0CG47)
| PDB | External Database | Details |
|---|---|---|
| Gly 387 | - | expression tag |
| Ser 388 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Lys 92 | Cys 92 | engineered mutation |
| Arg 97 | Lys 97 | engineered mutation |
| Cys 124 | Asp 124 | engineered mutation |
| Ser 170 | Cys 170 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Cys 48 | Lys 48 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 57641.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 351.0 | |
| All* | Total formula weight | 57992.7 |
