7MSG

The crystal structure of LIGHT in complex with HVEM and CD160

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97931
Spacegroup name	I 2 3
Unit cell lengths	214.689, 214.689, 214.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.933 - 3.500
Rwork	0.257
R-free	0.28470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rsu 6ng9
RMSD bond length	0.005
RMSD bond angle	1.290
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.830	34.830	3.830
High resolution limit [Å]	3.500	8.570	3.500
Rmerge	0.195	0.034	1.718
Rmeas	0.200	0.035	1.767
Rpim	0.044	0.008	0.414
Total number of observations		30804	88390
Number of reflections	20868	1474	4936
<I/σ(I)>	16.1	73.8	2.2
Completeness [%]	99.9	98.5	100
Redundancy	20.7	20.9	17.9
CC(1/2)	0.999	1.000	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	0.2 M potassium iodide, 20% PEG 3350