7MRU

Crystal structure of S62A MIF2 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-21
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.51418
Spacegroup name	P 21 21 21
Unit cell lengths	40.748, 75.891, 103.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.860 - 1.330
R-factor	0.1618
Rwork	0.161
R-free	0.18010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kan
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.350
High resolution limit [Å]	1.330	3.610	1.330
Rmerge	0.058	0.028	0.669
Rmeas	0.062	0.029	0.762
Rpim	0.022	0.008	0.348
Total number of observations	468497
Number of reflections	71882	4082	2350
<I/σ(I)>	11.2
Completeness [%]	95.1	100	63.3
Redundancy	6.5	11.9	3.7
CC(1/2)		1.000	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 N NaCl, 25%w/v PEG 3350, 0.1 M Tris at pH 8.5